Recombinant Human VEGF165 Protein
Recombinant Human VEGF165 Protein
- Stock: In Stock
- Model: GMP-TL612
$224.00
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VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; FMS-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
Cytokines | |
Expression Host | HEK293 Cells |
Synonym | MVCD1,VEGF,VEGF165,VPF |
Protein Sequence | A DNA sequence encoding the human VEGF165 (NP_001165097.1) was expressed with a polyhistidine tag at the C-terminus. |
Molecular Mass | The Recombinant Human VEGF165 consists of 193 amino acids and predicts a molecular mass of 22.3 kDa. |
QC Testing Purity | > 90 % as determined by SDS-PAGE. |
Endotoxin | < 0.1EU per μg of the protein as determined by the LAL method. |
Activity | Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED₅₀ for this effect is ≤20 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6% mannitol are added as protectants before lyophilization. |
Stability | 24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
Thumb | File information |
GMP-TL612_Product Sheet.pdf File Size81.55KB Downloaded: 81 | |
GMP-TL612_SDS.pdf File Size177.34KB Downloaded: 86 |