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Recombinant Human LIF Protein

Recombinant Human LIF Protein
Recombinant Human LIF Protein
  • Stock: In Stock
  • Model: GMP-TL633
$250.00

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LIF is a polytrophic factor produced by a variety of cell types, (including T cells, myeloid cell lines, fibroblasts, liver, heart, and melanoma). LIF promotes the long-term maintenance of embryonic stem cells by inhibiting their spontaneous differentiation. Other activities include hepatocyte stimulation of acute phase protein synthesis, stimulation of cholinergic nerve differentiation, and inhibition of lipogenesis by inhibiting lipoprotein lipase in adipocytes. Human LIF is active on mouse cells and is widely used to maintain the spontaneous differentiation of mouse ESC, while mouse LIF cannot function on human cells because it cannot bind to human LIF receptors. Recombinant Human LIF is a 19.6kDa protein with 180 amino acid residues and contains 3 disulfide bonds.

Cytokines
Expression Host HEK293 cells
Synonym CDF,DIA,HILDA,Leukemia Inhibitory Factor,MLPLI
Protein Sequence DNA sequence encoding human LIF(NP_002300.1) expressed with His tag at the C-terminus.
Molecular Mass The recombinant human LIF protein contains 186 amino acids and has a predicted molecular weight of 20.5kD.
QC Testing Purity > 90 % as determined by SDS-PAGE.
Endotoxin ≤0.01EU/ug (gel method).
Activity Determined by the expansion level of TF-1 cells. The expected ED₅₀ for this effect is ≤ 0.1ng/mL, corresponding to a specific activity of ≥1×10⁷U/mg.
Formulation
Stability 24 months at 4℃ in lyophilized state. 6 months-12 months at -20℃ under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Storage 24 months at 4℃ in lyophilized state. 6 months-12 months at -20℃ under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Thumb File information
GMP-TL633_SDS.pdf 161.82KB 84

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