Recombinant Human IL-21 Protein
- Stock: In Stock
- Model: GMP-TL509
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IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation.Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include:inducing the differentiation of T-cell-stimulated B-cells into plasma cells and memory B-cells; the stimulation of IgG production in conjunction with IL-4;and the induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally,IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells.IL-21 exerts its effect through binding to a specific type I cytokine receptor,IL-21R,which also contains the γ chain (γc) found in other cytokine receptors, including IL-2,IL-4,IL-7,IL-9 and IL-15.The IL-21/IL-21R interaction triggers a cascade of events,which includes activation of the tyrosine kinases JAK1 and JAK3,followed by activation of the transcription factors STAT1 and STAT3.
Cytokines | |
Expression Host | CHO cells |
Synonym | CVID11, IL-21, Za11 |
Protein Sequence | A DNA sequence encoding the extracellular domain of human IL-21(Q9HBE4-1: Q29-S162) was expressed with the C-terminal fused Fc region of human IgG1. |
Molecular Mass | The recombinant human IL-21 predicts a molecular mass of 41.9kD. |
QC Testing Purity | > 95 % as determined by SDS-PAGE. |
Endotoxin | < 0.01 EU per μg of the protein as determined by the LAL method. |
Activity | Measured by its ability of inducing human natural killer lymphoma NK-92 cells to secrete interferon-γ. The expected ED₅₀ is ≤ 50 ng/mL |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
Stability | Lyophilized preparation can be stored at -20 ℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
Thumb | File information |
GMP-TL509_SDS.pdf File Size162.53KB Downloaded: 84 | |
GMP-TL509_Product Sheet.pdf File Size84.22KB Downloaded: 102 |