Recombinant Human IL-12 Protein

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Model: GMP-TL508

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Tags: Immune Cell Culture

IL-12 is primarily produced by monocytes, macrophages, B cells and DCs. IL-12 is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40).IL-12 receptors are located mainly on T cells and NK cells, and IL-12 can stimulate Th1 and NK cell growth, while inhibiting Th2 cell responses. IL-12 augments cytotoxicity of T lymphocytes and NK cells through the enhancement of IFN-γ and TNF-α secretion, and inhibition of IL-4 production.

Cytokines
Expression Host	HEK293 cells
Synonym	IL12, p70, Interleukin-12
Protein Sequence	Recombinant human IL-12 is heterodimeric and composed of P29459-1 (IL12A) and P29460-1 (IL12B).
Molecular Mass	The Recombinant Human IL-12 Protein predicts a molecular mass of 65 kDa.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1 μg of the protein by the LAL method.
Activity	Measured by cell proliferation assay on PHA activated PBMC cells.The ED50 of this effect is ≤ 0.2 ng/mL.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.