Anti-Human CD28 mAb
Anti-Human CD28 mAb
- Stock: In Stock
- Model: GMP-TL102
$225.00
Available Options
The CD28 molecules express on the surface of most T cells and is considered to be unique to T cells. T cell reactivity to antigen is mainly mediated through the CD3 / TCR complex, but also depends on the synergy of other molecules. T cells can be efficiently activated by several pathways, including CD3 / TCR, CD2, and CD28. Mononuclear cells isolated from peripheral blood, bone marrow or cord blood can be the source of T cells proliferation in the presence of CD3, CD28 monoclonal antibodies and various other cytokines.
Monoclonal Antibodies | |
Applications | Soluble method: the concentration of 500 ng/mL is recommended, under which T cells can be activated and CD3⁺CD56⁺T cells can be expanded significantly. |
Biological activity | The binding rate with Jurkat cells shall not be less than 90%. |
Coating method: | 10 mL PBS buffer (5μg/mL anti-human CD3) is placed in a T75 flask at 4°C overnight. Please remove the PBS and wash the flask with saline gently three times before use. |
Endotoxin | < 0.01EU per μg of the protein as determined by the LAL method |
Expression Host | Mouse Hybridoma cells |
Formulation | Supplied as a 0.22μm filtered solution in PBS, PH 7.4. |
Purification: | Protein A purified from cell culture supernatant |
QC Testing Purity | > 95 % as determined by SDS-PAGE |
Stability & Storage | 24 months at 2℃ to 8℃. Avoid repeated freeze-thaw cycles. |
Thumb | File information |
GMP-TL102_SDS.pdf File Size92.22KB Downloaded: 122 | |
GMP-TL102_Product Sheet.pdf File Size81.4KB Downloaded: 97 |