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Recombinant Human TNFα Protein

 Recombinant Human TNFα Protein
Recombinant Human TNFα Protein
  • Stock: In Stock
  • Model: GMP-TL303
$336.00

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TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. TNF-α is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is involved in fighting against tumorigenesis. It is regarded as a potential protein of cancer treatment.


Cytokines
Expression Host HEK293 cells
Synonym DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
Protein Sequence A DNA sequence encoding the human TNF-α (GenBank: ADV31546.2 V77-L233) was expressed with a polyhistidine tag at the C- terminus.
Molecular Mass The Recombinant protein predicts a molecular mass of 18.2kDa.
QC Testing Purity > 90% as determined by SDS-PAGE.
Endotoxin < 0.1EU per μg of the protein as determined by the LAL method.
Activity Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of actinomycin D. The ED₅₀ for this effect is ≤5ng/mL.
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability 24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.
Thumb File information
GMP-TL303_SDS.pdf 154.26KB 112
GMP-TL303_Product Sheet.pdf 137.72KB 90

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